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st2  (R&D Systems)


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    R&D Systems st2
    Response of the phosphate buffered saline (PBS)-treated human peripheral blood basophils to IL-3 stimulation. Basophils were incubated on ice for 5 min with PBS. Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The forward and side scatter plot of the basophils immediately following PBS treatment and following 24-h culture in the presence of IL-3. ( B ) Histogram overlays showing the expression of basophil activation markers CD69, <t>ST2</t> and FcεRI in PBS-treated basophils cultured for 24 h in IL-3. Representative data from four donors are presented.
    St2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/st2/product/R&D Systems
    Average 92 stars, based on 11 article reviews
    st2 - by Bioz Stars, 2026-03
    92/100 stars

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    1) Product Images from "Acid Stripping of Surface IgE Antibodies Bound to FcεRI Is Unsuitable for the Functional Assays That Require Long-Term Culture of Basophils and Entire Removal of Surface IgE"

    Article Title: Acid Stripping of Surface IgE Antibodies Bound to FcεRI Is Unsuitable for the Functional Assays That Require Long-Term Culture of Basophils and Entire Removal of Surface IgE

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21020510

    Response of the phosphate buffered saline (PBS)-treated human peripheral blood basophils to IL-3 stimulation. Basophils were incubated on ice for 5 min with PBS. Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The forward and side scatter plot of the basophils immediately following PBS treatment and following 24-h culture in the presence of IL-3. ( B ) Histogram overlays showing the expression of basophil activation markers CD69, ST2 and FcεRI in PBS-treated basophils cultured for 24 h in IL-3. Representative data from four donors are presented.
    Figure Legend Snippet: Response of the phosphate buffered saline (PBS)-treated human peripheral blood basophils to IL-3 stimulation. Basophils were incubated on ice for 5 min with PBS. Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The forward and side scatter plot of the basophils immediately following PBS treatment and following 24-h culture in the presence of IL-3. ( B ) Histogram overlays showing the expression of basophil activation markers CD69, ST2 and FcεRI in PBS-treated basophils cultured for 24 h in IL-3. Representative data from four donors are presented.

    Techniques Used: Saline, Incubation, Cell Culture, Expressing, Activation Assay

    Response of the acetic acid buffer (pH 4)-treated human basophils to IL-3 stimulation. Basophils were incubated on ice for 5 min either with phosphate buffered saline (PBS) or ice-cold acetic acid buffer pH 4 (AA pH 4). Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The forward and side scatter plot of AA pH 4-treated basophils following 24-h culture in IL-3. ( B ) The yield of basophils (mean ± SD, n = 4 donors) after 24 h culture of basophils in IL-3. The values were calculated based on the percentage of cells in the P1 gate of forward and side scatter plot. ( C ) Histogram overlays showing the expression of basophil activation markers CD69, ST2 and FcεRI in AA pH 4-treated cells after 24 h culture in IL-3. Representative data from four donors are presented. ( D ) The expression (mean ± SD, n = 4 donors) of various activation markers (% positive cells or median fluorescence intensity, MFI) after 24 h culture of basophils in IL-3. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant; two-sided Students t-test (panel B) or one-way ANOVA with Tukey’s multiple comparison test (panel D).
    Figure Legend Snippet: Response of the acetic acid buffer (pH 4)-treated human basophils to IL-3 stimulation. Basophils were incubated on ice for 5 min either with phosphate buffered saline (PBS) or ice-cold acetic acid buffer pH 4 (AA pH 4). Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The forward and side scatter plot of AA pH 4-treated basophils following 24-h culture in IL-3. ( B ) The yield of basophils (mean ± SD, n = 4 donors) after 24 h culture of basophils in IL-3. The values were calculated based on the percentage of cells in the P1 gate of forward and side scatter plot. ( C ) Histogram overlays showing the expression of basophil activation markers CD69, ST2 and FcεRI in AA pH 4-treated cells after 24 h culture in IL-3. Representative data from four donors are presented. ( D ) The expression (mean ± SD, n = 4 donors) of various activation markers (% positive cells or median fluorescence intensity, MFI) after 24 h culture of basophils in IL-3. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant; two-sided Students t-test (panel B) or one-way ANOVA with Tukey’s multiple comparison test (panel D).

    Techniques Used: Incubation, Saline, Cell Culture, Expressing, Activation Assay, Fluorescence, Comparison

    Lactic acid treatment does not affect viability, yield and IL-3 induced activation of basophils, but removes only a small fraction of the cell surface bound IgE. Basophils were incubated on ice either with phosphate buffered saline (PBS) or ice-cold lactic acid buffer pH 3.9 (LA). Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The viability of cells immediately following LA treatment as analyzed by fixable viable dye staining. ( B ) The forward and side scatter plot of LA-treated basophils following 24-h culture in IL-3. ( C ) The expression of basophil activation markers ST2, FcεRI and CD69 in LA-treated cells after 24 h culture in IL-3 (mean ± SD, n = 4 experiments from two donors). ( D ) Efficacy of stripping of basophil surface-bound IgE by LA as analyzed by surface staining of IgE and analyses by flow cytometry. The intensity of IgE on the surface of basophils was represented by MFI values (Median fluorescence intensity). ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA with Tukey’s multiple comparison test.
    Figure Legend Snippet: Lactic acid treatment does not affect viability, yield and IL-3 induced activation of basophils, but removes only a small fraction of the cell surface bound IgE. Basophils were incubated on ice either with phosphate buffered saline (PBS) or ice-cold lactic acid buffer pH 3.9 (LA). Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The viability of cells immediately following LA treatment as analyzed by fixable viable dye staining. ( B ) The forward and side scatter plot of LA-treated basophils following 24-h culture in IL-3. ( C ) The expression of basophil activation markers ST2, FcεRI and CD69 in LA-treated cells after 24 h culture in IL-3 (mean ± SD, n = 4 experiments from two donors). ( D ) Efficacy of stripping of basophil surface-bound IgE by LA as analyzed by surface staining of IgE and analyses by flow cytometry. The intensity of IgE on the surface of basophils was represented by MFI values (Median fluorescence intensity). ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA with Tukey’s multiple comparison test.

    Techniques Used: Activation Assay, Incubation, Saline, Cell Culture, Staining, Expressing, Stripping Membranes, Flow Cytometry, Fluorescence, Comparison



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    Response of the phosphate buffered saline (PBS)-treated human peripheral blood basophils to IL-3 stimulation. Basophils were incubated on ice for 5 min with PBS. Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The forward and side scatter plot of the basophils immediately following PBS treatment and following 24-h culture in the presence of IL-3. ( B ) Histogram overlays showing the expression of basophil activation markers CD69, ST2 and FcεRI in PBS-treated basophils cultured for 24 h in IL-3. Representative data from four donors are presented.

    Journal: International Journal of Molecular Sciences

    Article Title: Acid Stripping of Surface IgE Antibodies Bound to FcεRI Is Unsuitable for the Functional Assays That Require Long-Term Culture of Basophils and Entire Removal of Surface IgE

    doi: 10.3390/ijms21020510

    Figure Lengend Snippet: Response of the phosphate buffered saline (PBS)-treated human peripheral blood basophils to IL-3 stimulation. Basophils were incubated on ice for 5 min with PBS. Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The forward and side scatter plot of the basophils immediately following PBS treatment and following 24-h culture in the presence of IL-3. ( B ) Histogram overlays showing the expression of basophil activation markers CD69, ST2 and FcεRI in PBS-treated basophils cultured for 24 h in IL-3. Representative data from four donors are presented.

    Article Snippet: Basophils were analyzed for the expression of FcεRIa (FcεRIa-FITC, clone CRA-1, Miltenyi Biotec, Paris, France), CD69 (CD69-APC/Cy7, Clone FN50, BD Biosciences, Le Pont de Claix, France), CD123 (CD123-BV421, clone 9F5, BD Biosciences), ST2 (ST2/IL-33R-PE polyclonal goat IgG, R&D Systems, Lille, France) and surface intensity of IgE (anti-IgE–APC, clone MB10-5C4, Miltenyi Biotec) by using LSR II flow cytometer (BD Biosciences) and data were analyzed by BD FACSDiva v8.0.1 (BD Biosciences) and FlowJo v10 softwares (FlowJo, LLC, Ashland, USA).

    Techniques: Saline, Incubation, Cell Culture, Expressing, Activation Assay

    Response of the acetic acid buffer (pH 4)-treated human basophils to IL-3 stimulation. Basophils were incubated on ice for 5 min either with phosphate buffered saline (PBS) or ice-cold acetic acid buffer pH 4 (AA pH 4). Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The forward and side scatter plot of AA pH 4-treated basophils following 24-h culture in IL-3. ( B ) The yield of basophils (mean ± SD, n = 4 donors) after 24 h culture of basophils in IL-3. The values were calculated based on the percentage of cells in the P1 gate of forward and side scatter plot. ( C ) Histogram overlays showing the expression of basophil activation markers CD69, ST2 and FcεRI in AA pH 4-treated cells after 24 h culture in IL-3. Representative data from four donors are presented. ( D ) The expression (mean ± SD, n = 4 donors) of various activation markers (% positive cells or median fluorescence intensity, MFI) after 24 h culture of basophils in IL-3. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant; two-sided Students t-test (panel B) or one-way ANOVA with Tukey’s multiple comparison test (panel D).

    Journal: International Journal of Molecular Sciences

    Article Title: Acid Stripping of Surface IgE Antibodies Bound to FcεRI Is Unsuitable for the Functional Assays That Require Long-Term Culture of Basophils and Entire Removal of Surface IgE

    doi: 10.3390/ijms21020510

    Figure Lengend Snippet: Response of the acetic acid buffer (pH 4)-treated human basophils to IL-3 stimulation. Basophils were incubated on ice for 5 min either with phosphate buffered saline (PBS) or ice-cold acetic acid buffer pH 4 (AA pH 4). Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The forward and side scatter plot of AA pH 4-treated basophils following 24-h culture in IL-3. ( B ) The yield of basophils (mean ± SD, n = 4 donors) after 24 h culture of basophils in IL-3. The values were calculated based on the percentage of cells in the P1 gate of forward and side scatter plot. ( C ) Histogram overlays showing the expression of basophil activation markers CD69, ST2 and FcεRI in AA pH 4-treated cells after 24 h culture in IL-3. Representative data from four donors are presented. ( D ) The expression (mean ± SD, n = 4 donors) of various activation markers (% positive cells or median fluorescence intensity, MFI) after 24 h culture of basophils in IL-3. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant; two-sided Students t-test (panel B) or one-way ANOVA with Tukey’s multiple comparison test (panel D).

    Article Snippet: Basophils were analyzed for the expression of FcεRIa (FcεRIa-FITC, clone CRA-1, Miltenyi Biotec, Paris, France), CD69 (CD69-APC/Cy7, Clone FN50, BD Biosciences, Le Pont de Claix, France), CD123 (CD123-BV421, clone 9F5, BD Biosciences), ST2 (ST2/IL-33R-PE polyclonal goat IgG, R&D Systems, Lille, France) and surface intensity of IgE (anti-IgE–APC, clone MB10-5C4, Miltenyi Biotec) by using LSR II flow cytometer (BD Biosciences) and data were analyzed by BD FACSDiva v8.0.1 (BD Biosciences) and FlowJo v10 softwares (FlowJo, LLC, Ashland, USA).

    Techniques: Incubation, Saline, Cell Culture, Expressing, Activation Assay, Fluorescence, Comparison

    Lactic acid treatment does not affect viability, yield and IL-3 induced activation of basophils, but removes only a small fraction of the cell surface bound IgE. Basophils were incubated on ice either with phosphate buffered saline (PBS) or ice-cold lactic acid buffer pH 3.9 (LA). Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The viability of cells immediately following LA treatment as analyzed by fixable viable dye staining. ( B ) The forward and side scatter plot of LA-treated basophils following 24-h culture in IL-3. ( C ) The expression of basophil activation markers ST2, FcεRI and CD69 in LA-treated cells after 24 h culture in IL-3 (mean ± SD, n = 4 experiments from two donors). ( D ) Efficacy of stripping of basophil surface-bound IgE by LA as analyzed by surface staining of IgE and analyses by flow cytometry. The intensity of IgE on the surface of basophils was represented by MFI values (Median fluorescence intensity). ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA with Tukey’s multiple comparison test.

    Journal: International Journal of Molecular Sciences

    Article Title: Acid Stripping of Surface IgE Antibodies Bound to FcεRI Is Unsuitable for the Functional Assays That Require Long-Term Culture of Basophils and Entire Removal of Surface IgE

    doi: 10.3390/ijms21020510

    Figure Lengend Snippet: Lactic acid treatment does not affect viability, yield and IL-3 induced activation of basophils, but removes only a small fraction of the cell surface bound IgE. Basophils were incubated on ice either with phosphate buffered saline (PBS) or ice-cold lactic acid buffer pH 3.9 (LA). Cells were then washed and cultured in serum-free X-VIVO 15 medium (0.1 × 10 6 cells/well per 200 µL) in 96-well U-bottomed plate along with IL-3 (100 ng/0.5 million cells) for 24 h. ( A ) The viability of cells immediately following LA treatment as analyzed by fixable viable dye staining. ( B ) The forward and side scatter plot of LA-treated basophils following 24-h culture in IL-3. ( C ) The expression of basophil activation markers ST2, FcεRI and CD69 in LA-treated cells after 24 h culture in IL-3 (mean ± SD, n = 4 experiments from two donors). ( D ) Efficacy of stripping of basophil surface-bound IgE by LA as analyzed by surface staining of IgE and analyses by flow cytometry. The intensity of IgE on the surface of basophils was represented by MFI values (Median fluorescence intensity). ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA with Tukey’s multiple comparison test.

    Article Snippet: Basophils were analyzed for the expression of FcεRIa (FcεRIa-FITC, clone CRA-1, Miltenyi Biotec, Paris, France), CD69 (CD69-APC/Cy7, Clone FN50, BD Biosciences, Le Pont de Claix, France), CD123 (CD123-BV421, clone 9F5, BD Biosciences), ST2 (ST2/IL-33R-PE polyclonal goat IgG, R&D Systems, Lille, France) and surface intensity of IgE (anti-IgE–APC, clone MB10-5C4, Miltenyi Biotec) by using LSR II flow cytometer (BD Biosciences) and data were analyzed by BD FACSDiva v8.0.1 (BD Biosciences) and FlowJo v10 softwares (FlowJo, LLC, Ashland, USA).

    Techniques: Activation Assay, Incubation, Saline, Cell Culture, Staining, Expressing, Stripping Membranes, Flow Cytometry, Fluorescence, Comparison